Journal: Journal of Proteome Research

Article Title: Top-Down Proteomics of a Drop of Blood for Diabetes Monitoring

doi: 10.1021/pr401074t

Figure Lengend Snippet: Validation of a top-down-proteomics-centric assay for diabetes monitoring. (a) Calibration curve for the ratio of HbA1c:HbA determined by our assay for known molar ratios of the mixtures of purified HbA1c and HbA0. (b) Comparison of HbA1c values for the Lyphochek Hemoglobin A1c linearity set samples (LOT 34650), obtained by our MEA chip-based assay (×), and the corresponding target values using other commercial assays (NGSP (⧫) and IFCC (○)) provided by Bio-Rad. Error bars, SD ( n ≥ 3) for our assay. (c, d) Comparison of HbA1c values for blood samples from T2D patients ( n = 8), each measured by our MEA chip and a commercial Tosoh G7 HPLC analyzer, respectively, showing the correlation between the values obtained by the two methods (Pearson’s correlation, r = 0.9695, p < 0.0001, n = 8) (c) and the Bland-Altman plot of the difference between the two methods. The lines were plotted indicating the bias (0.80%) and the upper and lower limits of agreement (LoA) (bias ±2 × SD) (d).

Article Snippet: Lyphochek hemoglobin A1c linearity set (lot no. 34650, level 1–4) was obtained from Bio-Rad (Hercules, CA).

Techniques: Biomarker Discovery, Purification, Comparison

Journal: Journal of Proteome Research

Article Title: Top-Down Proteomics of a Drop of Blood for Diabetes Monitoring

doi: 10.1021/pr401074t

Figure Lengend Snippet: Application of the assay for monitoring individual glycemia. (a, b, c) Quantitation of the glycation levels of HbA (HbA1c), HSA (GA), and apoA-I (GapoA-I) in controls and Type 2 diabetes (T2D) patients, respectively ( n = 8 for each group). The mean HbA1c, GA, and GapoA-I levels in T2D were 8.44%, 30.93%, and 5.10%, respectively, while in controls they were 5.95%, 17.05%, and 3.63%, respectively. The p values were calculated using the two-tailed Student’s t test. (d, e, f) Correlation between any two values of HbA1c, GA, and GapoA-I, respectively, for each individual monitored in (a–c) (Pearson’s correlation, p < 0.0001, n = 16).

Article Snippet: Lyphochek hemoglobin A1c linearity set (lot no. 34650, level 1–4) was obtained from Bio-Rad (Hercules, CA).

Techniques: Quantitation Assay, Two Tailed Test

Journal: Journal of Proteome Research

Article Title: Top-Down Proteomics of a Drop of Blood for Diabetes Monitoring

doi: 10.1021/pr401074t

Figure Lengend Snippet: Application of the assay for concurrently monitoring individual oxidative stress and cardiovascular risks. (a, b, c) Comparison of the levels of HSA cysteinylation (HSA-Cys), hemoglobin S-nitrosylation (HbA-SNO), and apoA-I oxidation (apoA-I MetO), between controls and T2D patients, respectively ( n = 8 for each group). The mean HSA-Cys, HbA-SNO, and apoA-I MetO levels in T2D were 49.59%, 19.33%, and 12.87%, respectively, while in controls they were 36.96%, 20.17%, and 12.01%, respectively. The p values were calculated using the two-tailed Student’s t test. (d) Correlation between the levels of HSA-Cys and GA (blue ●), HbA1c (green Δ), and GapoA-I (red ×), respectively, for each individual monitored in panels a–c (Pearson’s correlation, n = 16). (e) Correlation between the age of individual T2D patients and their levels of HSA-Cys (blue ●), HbA-SNO (green Δ), and apoA-I MetO (red ×), respectively (Pearson’s correlation, n = 8).

Article Snippet: Lyphochek hemoglobin A1c linearity set (lot no. 34650, level 1–4) was obtained from Bio-Rad (Hercules, CA).

Techniques: Comparison, Two Tailed Test

Journal: Journal of Proteome Research

Article Title: Top-Down Proteomics of a Drop of Blood for Diabetes Monitoring

doi: 10.1021/pr401074t

Figure Lengend Snippet: A scheme for rapid and multidimensional monitoring of diabetes using a drop of blood. Our top-down-proteomics-centric assay, enabled by our MEA chip platform, concurrently measures markers for multitime-scale glycemia (glucose, GapoA-I, GA, and HbA1c), oxidative stress (HSA-Cys), and cardiovascular risks (HbA-SNO, GapoA-I, and apoA-I MetO) in multiple compartments of blood, thereby contributing to better long-term monitoring and disease management of diabetes. The size of various components in the blood drop was not drawn to scale.

Article Snippet: Lyphochek hemoglobin A1c linearity set (lot no. 34650, level 1–4) was obtained from Bio-Rad (Hercules, CA).

Techniques: